BIOCHEMISTRY

CH 241                                                                      No. of hrs/week = 03 + 03

                                                                                    No. of credits  = 04

  1. Carbohydrate chemistry: monosaccharides, disaccharides and polysaccharides with examples and their significance                                                                                4hrs
  2. Lipid chemistry: Classification, with examples and significance                                    6hrs
  3. Amino acids and proteins-Classification on different basis, peptides, Classification of protein, Protein structure and denaturation, Importance of hemoglobin.    5hrs
  4. Enzymes- Classification and properties, importance of diagnostic enzymes.       3hrs
  5. Nucleic Acids: Types, structure of DNA and RNA, significance, recombinant DNA and its application.                                                                                                            4hrs
  6. Blood chemistry: Normal serum levels, clinical significance and estimations of glucose, urea, creatinine, protein, cholesterol and bilirubin.                                   3hrs
  7. Urine chemistry: Chemical composition of urine under normal and abnormal conditions.                                                                                                                5hrs
  8. Instrumentation: Principles and application of spectrophotometery, fluorimetry, flame photometry, serum protein electrophoresis and automation in clinical biochemistry. Measurement of pH using combination electrodes.                     5hrs
  9. Hormones: General introduction to hormones, classification and examples.     2hrs
  10. Metabolic pathways: Glycolysis, TCA cycle, b- oxidation, urea cycle    .           5hrs
  11. Bioenergetics                                                                                                                       3hrs

Practicals

  1. Carbohydrates : Reactions of mono, di and polysacchrides.

Identification of unknown carbohydrate.                                              4 practs

  1. Urine Analysis

Normal urine                                                                                                 1pr

Abnormal urine                                                                                              1pr

Analysis of unknown sample of urine                                                          1pr

      3.  Estimation of serum glucose                                                                          1pr

      4.  Estimation of  urine creatinine                                                                      1pr