BIOCHEMISTRY

 

CH 241                                                                                  No. of hrs/week = 03 + 03

                                                                                                No. of credits  = 04

 

  1. Carbohydrate chemistry: monosaccharides, disaccharides and polysaccharides with examples and their significance                                                                             4hrs
  2. Lipid chemistry: Classification, with examples and significance                             6hrs
  3. Amino acids and proteins-Classification on different basis, peptides, Classification of protein, Protein structure and denaturation, Importance of hemoglobin.                     5hrs
  4. Enzymes- Classification and properties, importance of diagnostic enzymes.         3hrs
  5. Nucleic Acids: Types, structure of DNA and RNA, significance, recombinant DNA and its application.                                                                                                                 4hrs
  6. Blood chemistry: Normal serum levels, clinical significance and estimations of glucose, urea, creatinine, protein, cholesterol and bilirubin.                                            3hrs
  7. Urine chemistry: Chemical composition of urine under normal and abnormal conditions.                                                                                                             5hrs
  8. Instrumentation: Principles and application of spectrophotometery, fluorimetry, flame photometry, serum protein electrophoresis and automation in clinical biochemistry. Measurement of pH using combination electrodes.                                                      5hrs
  9. Hormones: General introduction to hormones, classification and examples.          2hrs
  10. Metabolic pathways: Glycolysis, TCA cycle, b- oxidation, urea cycle         .           5hrs
  11. Bioenergetics                                                                                                           3hrs

 

Practicals

 

  1. Carbohydrates : Reactions of mono, di and polysacchrides.

Identification of unknown carbohydrate.                                                          4 pr

  1. Urine Analysis

Normal urine                                                                                                   1pr

Abnormal urine                                                                                                1pr

Analysis of unknown sample of urine                                                 1pr

      3.  Estimation of serum glucose                                                                           1pr

      4.  Estimation of  urine creatinine                                                                         1pr